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Bacterial testing of root canal systems in patients has traditionally presented with many difficulties. The culture technique never gained wide acceptance as the procedure is arduous and time consuming. DNA-based identification such as polymerase chain reaction suffers from high false positive readings by detecting DNA from dead bacterial cells. Extrapolating data from research in extracted teeth, with artificially created biofilm, to the clinical treatment of patients is fraught with potential miscalculations and erroneous inferences. While this research can provide valuable information as to the efficacy of different endodontic protocols, it does not take in to account the restrictions encountered while treating patients.
A newly introduced ATP assay method provides rapid identification of organic residues in root canal systems by detecting ATP, as well as its degradation products ADP and AMP, using a combination of luminescent reactions. The resulting luminescence is measured within 10 seconds and displayed as a 0 – 100 Endoscore, with low scores indicating less cellular (bacterial) contamination.
The rapid identification of biologic activity within canals not only allows dentists in real time to assess, and if necessary, repeat or modify their endodontic protocol, but it also is an accurate indicator of the readiness of the root canal system to be obturated at that visit.
This presentation will review the ATP assay detection method and present data validating an Er,Cr:YSGG laser-assisted endodontic protocol for the cleaning and disinfection of root canal systems. Clinical cases will be presented illustrating the role that the ATP assay test can play in the management of different clinical situations.
Release date: 2025-02-13 | Expiration date: 2028-02-13
Tribune Group GmbH is an ADA CERP Recognized Provider.
ADA CERP is a service of the American Dental Association to assist dental professionals in identifying quality providers of continuing dental education. ADA CERP does not approve or endorse individual courses or instructors, nor does it imply acceptance of credit hours by boards of dentistry. This continuing education activity has been planned and implemented in accordance with the standards of the ADA Continuing Education Recognition Program (ADA CERP) through joint efforts between Tribune Group GmbH and Dental Tribune International GmbH, recognized through ADA CERP from 5/1/24 - 6/30/27.
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Please use this link to get your CE: https://www.biolaseacademy.com/webinar/validation-of-an-ercrysgg-laser-assisted-endodontic-protocol-using-a-rapid-real-time-atp-assay-test/?sb=ce-certificate
Thank you!
Thank you
quiz
thank you
How do receive credit for this course
I tried the preset iplus irrigation protocol laser er cr ysgg with sodium chloride. But when i start to activate with the laser the sodium chloride gone from the canal. Is because the too much air or? Any idea to solve the problem?
Thank you for this great webinar.
Would you recommend using an RFT5 tip to activate the NaOCL above the canal entrance instead of an RFT3 tip?
can you please explain again the desinfection on hte orifice af the canal? What effects do you appreciate that are taking place?
Thank you from one of your referrers who is consistently astounded by the quality of your work and the compassion you show to our patients
thank you. would you be able to give the settings for conditioning cleaning and disinfection?
What is the correlation ATP with inflammatory mediators
Thank you so much
Thanks for a great lecture from Terry in Cape Town!
Thanks,doc.
Thank You
That was amazing cases
So you prefer ceramic sealers prof.?
Would you recommend using a dual wavelength disinfection protocol with a diode laser eg 940 nm rather than using sod. hypochlorite? Has the ATP test been carried out using a dual wavelength protocol for comparison?
what type of needle irrigation tip do you use with naOcl laser activication ?
Is there any faster procedures ?
What is you opinion after all your experience, what about saline, distilled water as the irrigation after and between sodium hypochlorite and about the case like age the root apex development widening with laser?
Greetings from Qatar
Thank you for joining today’s webinar with Dr. Justin Kolnick. If you have any questions about today’s presentation, please write them in this chat box and they will be addressed during the Q&A session.